送交者: 红旗飘飘 于 2006-1-12, 06:46:43:
回答: 如果克隆序列有翻译开始信号，是可以用来表达的，但是从该论文公布的克隆序列看，没有 由 方舟子 于 2006-1-12, 02:18:51:
The plasmid was made? by P. Ghosh, who solved the structure of colicin Ia then years ago (published in Nature). A promoter and SD sequence was introduced into the plasmid.